ABSTRACT
<p><b>OBJECTIVE</b>To explore the molecular mechanism of realgar-induced apoptosis of cervical cancer cells.</p><p><b>METHODS</b>The cervical cancer cell line Siha was used to determine the cell viability and apoptosis after treatment with realgar using MTT assay and flow cytometry. The activities of caspase-3, -8, and -9 were detected by fluorescence resonance energy transfer technology and colorimetric assay, while the levels of Bcl-2, cytochrome c, and Bax were detected by Western blot method.</p><p><b>RESULTS</b>Induction of apoptosis by realgar was detected in Siha cell line in a dose-dependent manner. The apoptosis was accompanied by a significant increase in cytochrome c release and activation of caspase-3 and caspase-9 but not caspase-8. Further, the realgar-induced apoptosis was inhibited by a broad-spectrum caspase inhibitor, a caspase-3 inhibitor, and a caspase-9 inhibitor but not by a caspase-8 inhibitor. Bcl-2 and Bax protein expressions were not changed by realgar.</p><p><b>CONCLUSION</b>The induction of apoptosis by realgar is mediated through a cytochrome c-dependent pathway, which sequentially activates caspase-9 and caspase-3.</p>
Subject(s)
Female , Humans , Apoptosis , Physiology , Arsenicals , Pharmacology , Caspase 3 , Metabolism , Caspase 8 , Metabolism , Caspase 9 , Metabolism , Caspase Inhibitors , Cell Line, Tumor , Cell Survival , Physiology , Cytochromes c , Metabolism , Dose-Response Relationship, Drug , Enzyme Activation , Enzyme Inhibitors , Pharmacology , Fluorescence Resonance Energy Transfer , Sulfides , Pharmacology , Uterine Cervical Neoplasms , Drug Therapy , Metabolism , PathologyABSTRACT
To report our experience on the erosion of polypropylene mesh used in pelvic reconstructive surgeries, and to discuss the pathological changes of mesh erosion. We conducted a retrospective study of 128 patients receiving pelvic reconstructive surgeries with polypropylene mesh from May 2006 to May 2009 in the Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Hubei, China. Data regarding patient demographics, operation notes, and follow up were collected. The mean follow-up time for the 128 patients was 15.2 [1.3-60] months. Seven patients were diagnosed with vaginal mesh erosion [Prolene mesh], and one of them suffered from anaphylactoid breakout related to the mesh. The mean [ +/- standard deviation] time occurring in the mesh erosion was 9.1 +/- 7.6 months. All the 7 patients were treated with surgery. Chronic inflammation was the main pathological manifestations in the eroded tissue. Most cases of polypropylene mesh erosions occur within one year postoperatively. Removal of the mesh could be the best therapy for mesh erosion. Further study is needed to determine if the polypropylene mesh induces supersensitivity